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Urinary Microbiota; Which Non-invasive Urine Collection Method Should We Use?
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  • Muhammed Selcuk Ozer,
  • Hüseyin Alperen Yıldız,
  • Canet Incir,
  • Dogan Deger,
  • Ozan Bozkurt,
  • Gül Ergör,
  • Yesim Tuncok,
  • Nuran Esen,
  • Ahmet Adil Esen
Muhammed Selcuk Ozer
Dokuz Eylul University Faculty of Medicine
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Hüseyin Alperen Yıldız
Malazgirt State Hospital
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Canet Incir
Dokuz Eylul University Faculty of Medicine
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Dogan Deger
Edirne Sultan Murat 1 State Hospital
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Ozan Bozkurt
Dokuz Eylul University Faculty of Medicine
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Gül Ergör
Dokuz Eylul University Faculty of Medicine
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Yesim Tuncok
Dokuz Eylul University Faculty of Medicine
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Nuran Esen
Dokuz Eylul University Faculty of Medicine
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Ahmet Adil Esen
Dokuz Eylul University Faculty of Medicine
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Abstract

Objective: The aim of this study is establish the optimal non- invaszive urine sample collection method for the microbiota studies. Methodology: 12 men with bladder carcinoma underwent first voided and midstream urine collection. Urine samples were analyzed by using V3-V4 regions of bacterial 16s ribosomal RNAs. Bacterial groups with relative abundance above 1% were analyzed in first voided urine and midstream urine samples at phylum, class, order, and family level. At the genus level, all of the identified bacterial groups’ relative abundances were analyzed. The statistical significance (p<0.05) of differences between first voided and midstream urine sample microbiota were evaluated using the Wilcoxon test. Results: According to analysis, 8 phyla, 14 class, 23 orders, 39 families, and 29 different genera were identified in the first voided and the midstream urine samples. Statistical differences were not identified between first voided and mid-stream urine samples of all bacteria groups except the Clostridiales at order level (p:0.04) and Clostridia at class level (p:0.04). Conclusions: Either first voided or midstream urine samples can be used in urinary microbiota studies as we determined that there is no statistically significant difference between them regarding the results of 16s ribosomal RNA analysis. What’s known? According to widespread acceptance, first voided urine and midstream urine should be collected separately for standard microbiologic evaluation. What’s new? We found that there is no exact statistically significant difference between two collection methods even on microbiota analysis. We believe that either first voided or midstream uyrine samples can be used in urinary microbiota studies.

Peer review status:ACCEPTED

13 Feb 2021Submitted to International Journal of Clinical Practice
15 Feb 2021Submission Checks Completed
15 Feb 2021Assigned to Editor
20 Feb 2021Reviewer(s) Assigned
21 Feb 2021Review(s) Completed, Editorial Evaluation Pending
17 Mar 20211st Revision Received
18 Mar 2021Submission Checks Completed
18 Mar 2021Assigned to Editor
25 Mar 2021Reviewer(s) Assigned
25 Mar 2021Review(s) Completed, Editorial Evaluation Pending
27 Mar 2021Editorial Decision: Accept