Dynamic thiol-disulfide balance and thioredoxin reductase enzyme levels
in patients with chronic kidney disease.
Introduction: We aimed to measure the dynamic thiol-disulfide balance
and thioredoxin reductase (TrxR) enzyme levels in patients with chronic
kidney disease (CKD). Material and Methods: Thirty hemodialysis (HD), 30
CKD patients (stage3-5) and 30 controls were included in the study. The
dynamic thiol-disulfide balance was determined by the colorimetric
method developed by Erel et al. TrxR levels were determined by ELISA.
Results: Native and total thiol levels of CKD and HD patients were
significantly lower than that of the control group (p=0.001for both).
However, disulfide levels were significantly higher in the HD group
(p=0.001), but there was no significant difference between control and
CKD groups(p=0.547). A notable negative correlation was found between
the native and total thiol levels and IMA(r=-0.628;-0.631),BUN
(r=-0.747;-0.747),and creatinine(r=-0.732;-0.721). There was a
significant positive correlation between GFR and the thiol levels
(r=0.835;0.824). TrxR levels were significantly higher in the patient
groups compared to the controls (p=0.001).CRP levels of the patient
groups were significantly higher compared to the controls (p=0.001).
Conclusions: We have demonstrated that measurement of dynamic
thiol-disulfide levels by using colorimetric method can contribute to
the diagnosis and follow-up of the disease as a marker, because, it is
easily applicable in routine clinical biochemistry laboratories and
related with disease severity in CKD patients. Also, we showed that
volume correction due to dialysis process should be consider in studies
dealing with plasma thiol values and the final results should be given
after the correction process.