loading page

Dynamic thiol-disulfide balance and thioredoxin reductase enzyme levels in patients with chronic kidney disease.
  • +2
  • Huseyin Erdal,
  • Oguzhan Ozcan,
  • Faruk Turgut,
  • Salim Neselioglu,
  • Ozcan Erel
Huseyin Erdal
Aksaray University
Author Profile
Oguzhan Ozcan
Hatay Mustafa Kemal University
Author Profile
Faruk Turgut
Hatay Mustafa Kemal Universitesi
Author Profile
Salim Neselioglu
Yildirim Beyazit University Faculty of Medicine
Author Profile
Ozcan Erel
Yildirim Beyazit University Faculty of Medicine
Author Profile

Abstract

Introduction: We aimed to measure the dynamic thiol-disulfide balance and thioredoxin reductase (TrxR) enzyme levels in patients with chronic kidney disease (CKD). Material and Methods: Thirty hemodialysis (HD), 30 CKD patients (stage3-5) and 30 controls were included in the study. The dynamic thiol-disulfide balance was determined by the colorimetric method developed by Erel et al. TrxR levels were determined by ELISA. Results: Native and total thiol levels of CKD and HD patients were significantly lower than that of the control group (p=0.001for both). However, disulfide levels were significantly higher in the HD group (p=0.001), but there was no significant difference between control and CKD groups(p=0.547). A notable negative correlation was found between the native and total thiol levels and IMA(r=-0.628;-0.631),BUN (r=-0.747;-0.747),and creatinine(r=-0.732;-0.721). There was a significant positive correlation between GFR and the thiol levels (r=0.835;0.824). TrxR levels were significantly higher in the patient groups compared to the controls (p=0.001).CRP levels of the patient groups were significantly higher compared to the controls (p=0.001). Conclusions: We have demonstrated that measurement of dynamic thiol-disulfide levels by using colorimetric method can contribute to the diagnosis and follow-up of the disease as a marker, because, it is easily applicable in routine clinical biochemistry laboratories and related with disease severity in CKD patients. Also, we showed that volume correction due to dialysis process should be consider in studies dealing with plasma thiol values and the final results should be given after the correction process.