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Human BLK tyrosine protein kinase is closely related to Pongo Albelli: An in silico structural and functional analysis
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  • Princy Princy,
  • Shaban AhmadOrcid,
  • Fatima Khan,
  • Sahil Mehta,
  • Sahar Qazi,
  • Khalid Raza
Princy Princy
Indian Agricultural Research Institute
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Shaban Ahmad
Orcid
Indian Agricultural Research Institute
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Fatima Khan
NCERT
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Sahil Mehta
ICGEB New Delhi
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Sahar Qazi
Jamia Millia Islamia
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Khalid Raza
Jamia Millia Islamia, Jamia Millia Islamia
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Abstract

BLK belongs to the family of SRC kinases (SFKs), and are diagnosed with the aid of the presence of an SH3 and SH2 regulatory domains of N-terminal to the catalytic kinase domain. BLK in signalling has a vast position in transmitting alerts via immunoglobulins and ends in pro-B to pre-B conversion, and in signalling for boom arrest and apoptosis downstream of the B-cellular receptor. We have performed a series of computational analysis on various aspects on BLK viz phylogenetic analysis, domain analysis, secondary structure prediction, charge distribution, prediction of the antigenic region and have executed structural analysis by first structure modelling and then its refinement, and active site prediction for better understanding of the human BLK as a drug target. Our study includes a detailed analysis and graphical representation of different domains, charge distribution, prediction of the antigenic region etc with corresponding sequence and its secondary structure for the pharmacological aspect of BLK which observed that Tulipa suaveolens is the most outed clade in the BLK and Human BLK is found to be very closed to Pongo Abelii through the phylogenetic tree assessment.