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Lipopolysaccharide-activated bone marrow-derived dendritic cells suppress ovalbumin(OVA)-induced asthma by ameliorating the immune microenvironment
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  • Zhihui Min,
  • Yuzhen Zeng,
  • Tao Zhu,
  • Bo Cui,
  • Ruolin Mao,
  • Meiling Jin,
  • Zhihong Chen
Zhihui Min
Zhongshan Hospital Fudan University
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Yuzhen Zeng
Zhongshan Hospital Fudan University
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Tao Zhu
Second Affiliated Hospital of Chongqing Medical University
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Bo Cui
Zhongshan Hospital Fudan University
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Ruolin Mao
Zhongshan Hospital Fudan University
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Meiling Jin
Zhongshan Hospital Fudan University
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Zhihong Chen
Zhongshan Hospital Fudan University
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Abstract

LPS produced by Gram-negative bacteria effectively stimulates the maturation of BMDCs. Previous studies have shown that DClps might induce tolerance in autoimmune diseases and cancer in vivo, whereas it remains unclear whether DClps can modulate the immune microenvironment in allergic asthma. We sought to elucidate the potential effects of DClps on OVA-sensitized/challenged airway inflammation in a mouse model of asthma, which may help facilitate the application of specific tolDCs in allergic asthma patients in the future. We generated and obtained DClps from wild-type mice to evaluate their functional characteristics by ELISA and FACS. We also induced OVA-sensitized/challenged asthmatic mice and intraperitoneally treated these mice with DClps to assess the effects of these injected cells by histopathologic analysis and performing inflammatory cell counts in BALF. Changes in memory CD4+ T cells, Tregs and phosphorylated protein in lung digests were analyzed. DClps exhibited lower levels of CD80 and MHCII and increased levels of anti-inflammatory cytokines such as IL-10 and TGF-β than DCia. Additionally, DClps treatment dramatically ameliorated airway inflammation and diminished the infiltration of pulmonary inflammatory cells. In addition, we prolonged the modeling time of asthmatic mice and demonstrated that DClps treatment decreased the proliferation activity of pulmonary memory CD4+ T cells, which further rendered the downregulation of Th2 cytokines. However, the number of pulmonary Tregs did not discernibly change. DClps treatment also markedly reduced the phosphorylation level of STAT6 protein.