Background Dietary inflammatory index (DIP) is a new dietary index designed to evaluate individuals’ diets. In addition, adhesion molecules are important biomarkers for assessing endothelium inflammation that they related to atherosclerosis and cardiovascular disease. Also, there is no study for assessing the association between adhesion molecules and DIP until now as well as other studies that assessed the relationship between dietary inflammatory index or DIP have controversy. Aim of the Work The purpose of this cross-sectional study was to determine the correlation between DII and endothelial markers such as E-selectin, intercellular adhesion molecule-1 (sICAM-1) and vascular cell adhesion molecule-1 (sVCAM-1) among female nurses from Isfahan. In this study, dietary inflammatory potential (DIP) was used instead of DII. Patients and Methods This study was performed on 420 healthy nurses. The nurses were selected by random cluster sampling method from private and public Isfahan hospitals. A validated food frequency questionnaire (FFQ) was applied to assess the dietary inflammatory potential. A fasting blood sample was collected for measuring the plasma levels of the endothelial markers and other variables. Results After adjusting different potential confounders, no statistical association was found between DIP and sICAM-1, E–selectin and sVCAM-1 in model I (P=0.57, 0.98 and 0.45), model II (P=0.57, 0.98 and 0.45) and model III (P=0.67, 0.92 and 0.50) in comparison to the crude group (P=0.35, 0.83 and 0.49, respectively). Conclusion The results revealed that the plasma levels of endothelial markers including E-selectin, sICAM-1, and sVCAM-1 were not significantly associated with DIP in female nurses.