Introduction: The primary aim of this study is to investigate the histopathologic effects of acute pioglitazone intoxication and the effects of intralipid emulsion treatment in reversing these possible unwanted effects in a rat model. Methods: For the study, 32 Spraque-Dawley rats weighing between 250-300 g were randomly divided into 4 groups. The first group was the control group and 12.4 ml/kg normal saline was given by intravenously (IV). 12.4 ml/kg intralipid emulsion was given to the second group by IV route. ½ LD50 pioglitazone (1000 mg/kg) per oral (PO) followed by 12.4 ml/kg normal saline IV was given to the third group. ½ LD50 pioglitazone (1000 mg / kg) PO followed by 12.4 ml / kg intralipid emulsion IV was then given to the fourth group. After 24 hours, rats were decapitated and tissue samples were taken for histopathological examination. Results: The histopathological scores of liver tissue in the third and fourth groups were significantly higher than the other groups. The histopathological scores of the kidney tissue were significantly higher in the second, third and fourth groups compared to the control group. The histopathological scores of the heart tissue were examined, no statistically significant difference was found in our model. ILE itself were found toxic to kidney cells. Conclusion: Although toxic effects can be observed in the liver and kidney tissues due to pioglitazone usage, intralipid emulsion treatment is insufficient to reverse these toxic effects. Toxic effects can be seen in the kidney tissue due to intralipid treatment and studies on different doses and timing are needed. Keywords: Pioglitazone, intravenous lipid emulsion, intoxication, animal model