Multiple-omics techniques reveal the difference between polyphagous
Conogethes punctiferalis and oligophagous C. pinicolalis
Abstract
Although Conogethes punctiferalis and Conogethes pinicolalis have
reported on morphology and molecular identification, the molecular
biology differences between the two species in different diets are still
unknown. In this work, we investigated the difference in the biological
process of the two species by the methods of transcriptomics,
proteomics, metabolomics, and bioinformatics. The results showed that
the expression of 74,611 mRNA in the transcriptome, 391 proteins in the
proteome, and 218 metabolites in the metabolome had been significantly
changed between the two species, and the KEGG results showed the data
were mainly closely related to metabolism and redox. Moreover, based on
integrating system-omics data, we found that the α-amylase and CYP6AE76
gene have mutations between the two species. Different sequence analysis
results may differ significantly in their exact substrate preference and
product profile, which indirectly leads to the different detoxification
or metabolism ability, and the gene expression of relevant was also
confirmed by the qPCR and enzyme activity test. These findings in the
two species and integrated networks provide beneficial information for
further exploring the molecular mechanism of polyphagous and
oligophagous.