Construction and characterization of a full-length cDNA infectious clone
of emerging porcine Teschovirus-2
Abstract
Porcine teschovirus (PTV) is a causative agent of polio,
encephalomyelitis, reproductive disorders, and gastrointestinal and
respiratory diseasesin swine. In this study, the PTV-2 GX/2020 strain
was isolated from pig intestinal tissue using ST cells. Phylogenetic
analysis of VP1 nucleotide sequences indicated that the GX/2020 strain
was closely related to PTV-2 (86%). Furthermore, a full-length cDNA
infectious clone of GX/2020 was constructed using seamless ligation
technology. The rescued virus was had a genome sequence largely
consistent with the parental virus sequence and had similar growth
characteristics. Overall, the PTV-2 virus successfully isolated in this
study and the employed reverse genetic platform have laid a foundation
for studying the pathogenic mechanism of porcine Teschovirus.